新城疫疫苗毒和低致病性禽流感病毒感染SPF鸡炎症因子的变化

doi:10.11843/j.issn.0366-6964.2015.04.019

Abstract

Abstract:

The aim of the present study was to investigate if the changes of inflammatory cytokines (interleukin 1，interleukin 6，interleukin 8，tumor necrosis factor alpha) and tracheal washing liquid and trachea mucosal secretory immunglobulin A (SIgA) can be used as judgments or warning indicators of respiratory diseases.We established a respiratory diseases model by infecting SPF chickens with Newcastle disease LaSota live vaccine and low pathogenic influenza virus (H9N2) single or mixed.Inflammatory factors of serum and SIgA were measured by double antibody sandwich ELISA method to analyze the inner relationship between the quantity changes and respiratory diseases.The results showed that SPF chickens appeared respiratory symptoms after inoculating LaSota vaccine and H9N2 AIV，content of inflammatory factors and mucosal antibody became significantly higher than those in control group，the changes of five cytokines content induced by two viral infection had obvious differences with time going，inflammation mediums became roughly same while the content in serum were less than in washing liquid.Pathological changes of chicken respiratory tract infection were closely related to the changing process of five inflammatory factors.With the help of detection of inflammatory factors，especially the IL-8，the severity of respiratory tract inflammation could be identified，so the detection of its content would have important reference value for the judgment of respiratory illness and early warning.

CLC Number:

S852.4

NIU Yu-juan，MA Hai-ying，ZHANG Zhen-dong，Lü Chuan-wei，ZHU Ming-xia，LIU Si-dang. The Changes of Inflammatory Factors in SPF Chicken infected by LaSota live Vaccine and H9N2 Influenza Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 644-649.

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The Changes of Inflammatory Factors in SPF Chicken infected by LaSota live Vaccine and H9N2 Influenza Virus

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